Brazilian Center for Research in energy and materials (CNPEM)

2 ARTICLES PUBLISHED IN JoVE

Environment

A Flexible Low Cost Hydroponic System for Assessing Plant Responses to Small Molecules in Sterile Conditions

Carolina C. Monte-Bello^*1,2, Elias F. Araujo^*1,3, Marina C.M. Martins¹, Valeria Mafra⁴, Viviane C.H. da Silva^1,2, Viviane Celente¹, Camila Caldana^1,5

¹Brazilian Bioethanol Science and Technology Laboratory (CTBE), Brazilian Center for Research in energy and materials (CNPEM), ²University of Campinas (UNICAMP), ³University of Viçosa (UFV), ⁴CTBE, CNPEM, ⁵Brazilian Bioethanol Science and Technology Laboratory (CTBE/CNPEM), Max Planck Partner Group

A simple, versatile, and low-cost in vitro hydroponic system was successfully optimized, enabling large-scale experiments under sterile conditions. This system facilitates the application of chemicals in a solution and their efficient absorption by roots for molecular, biochemical, and physiological studies.

JoVE Core

An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment

Talita Miguel Marin¹, Nathalia de Carvalho Indolfo⁴, Silvana Aparecida Rocco¹, Murilo de Carvalho^1,2, Marilia Meira Dias¹, Graziele Izalina Vasconcelos Bento¹, Leandro Oliveira Bortot¹, Desirée Cigaran Schuck³, Márcio Lorencini³, Eduardo Pagani¹

¹Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), ²Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), ³Grupo Boticário, ⁴Institute of Biology, University of Campinas

We exposed a microphysiological system (MPS) with intestine and liver organoids to acetaminophen (APAP). This article describes the methods for organoid production and APAP pharmacokinetic and toxicological property assessments in the MPS. It also describes the tissue functionality analyses necessary to validate the results.