Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus of TU Dresden
2 ARTICLES PUBLISHED IN JoVE
Biology
Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets
Sharan Janjuha 1,2, Sumeet Pal Singh 1, Nikolay Ninov 1,2
1Center for Molecular and Cellular Bioengineering, TU Dresden, 2Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus of TU Dresden
Beta-cell functionality is important for blood-glucose homeostasis, which is evaluated at single-cell resolution using a genetically encoded reporter for calcium influx.
Developmental Biology
Simultaneous Calcium Imaging and Glucose Stimulation in Living Zebrafish to Investigate In Vivo β-Cell Function
Luis Fernando Delgadillo-Silva 1, Mohammad Nadeem Akhtar 1, Emirhan Taşöz 1, Nikolay Ninov 1,2
1Centre for Regenerative Therapies TU Dresden, 2German Center for Diabetes Research (DZD e.V.), Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus of TU Dresden
Here, the study presents a protocol for calcium imaging and glucose stimulation of the pancreatic β-cells of the zebrafish in vivo.
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