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Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus of TU Dresden

2 ARTICLES PUBLISHED IN JoVE

Biology

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

Sharan Janjuha^1,2, Sumeet Pal Singh¹, Nikolay Ninov^1,2

¹Center for Molecular and Cellular Bioengineering, TU Dresden, ²Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus of TU Dresden

Beta-cell functionality is important for blood-glucose homeostasis, which is evaluated at single-cell resolution using a genetically encoded reporter for calcium influx.

Developmental Biology

Simultaneous Calcium Imaging and Glucose Stimulation in Living Zebrafish to Investigate In Vivo β-Cell Function

Luis Fernando Delgadillo-Silva¹, Mohammad Nadeem Akhtar¹, Emirhan Taşöz¹, Nikolay Ninov^1,2

¹Centre for Regenerative Therapies TU Dresden, ²German Center for Diabetes Research (DZD e.V.), Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus of TU Dresden

Here, the study presents a protocol for calcium imaging and glucose stimulation of the pancreatic β-cells of the zebrafish in vivo.