Texas A&M Agrilife Center at Dallas

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes

Jeanmarie Verchot¹, Aastha Thapa², Dulanjani Wijayasekara³, Peter R. Hoyt⁴

¹Texas A&M Agrilife Center at Dallas, ²Noble Research Center, Oklahoma State University, ³Department of Biology, College of Engineering and Natural Sciences, The University of Tulsa, ⁴Bioinformatics and Genomics Core Facility, Department of Biochemistry and Molecular Biology, Oklahoma State University

Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups.