Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus GenomesJeanmarie Verchot 1, Aastha Thapa 2, Dulanjani Wijayasekara 3, Peter R. Hoyt 4
1Texas A&M Agrilife Center at Dallas, 2Noble Research Center, Oklahoma State University, 3Department of Biology, College of Engineering and Natural Sciences, The University of Tulsa, 4Bioinformatics and Genomics Core Facility, Department of Biochemistry and Molecular Biology, Oklahoma State University
Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups.
