Greater Los Angeles Veteran Administration Healthcare System

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

Multimodal Bioluminescent and Positronic-emission Tomography/Computational Tomography Imaging of Multiple Myeloma Bone Marrow Xenografts in NOG Mice

Gilbert Gastelum¹, Eric Y. Chang², David Shackleford³, Nicholas Bernthal³, Jeffery Kraut^1,3, Kevin Francis⁴, Victoria Smutko¹, Patrick Frost^1,3

¹Greater Los Angeles Veteran Administration Healthcare System, ²San Diego Veterans Administration Healthcare System, ³University of California, Los Angeles, ⁴Perkin Elmer

Here we use bioluminescent, X-ray, and positron-emission tomography/computed tomography imaging to study how inhibiting mTOR activity impacts bone marrow-engrafted myeloma tumors in a xenograft model. This allows for physiologically relevant, non-invasive, and multimodal analyses of the anti-myeloma effect of therapies targeting bone marrow-engrafted myeloma tumors in vivo.