Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models

Amélie Slembrouck-Brec^*1, Céline Nanteau^*1, José-Alain Sahel¹, Olivier Goureau¹, Sacha Reichman¹

¹Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France

The production of specialized retinal cells from pluripotent stem cells is a turning point in the development of stem cell-based therapy for retinal diseases. The present paper describes a simple method for an efficient generation of retinal organoids and retinal pigmented epithelium for basic, translational, and clinical research.

Developmental Biology

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

Karim Ben M'Barek^1,2,3, Walter Habeler^1,2,3, Alexandra Plancheron^1,2,3, Mohamed Jarraya⁴, Olivier Goureau⁵, Christelle Monville^1,2

¹U861, I-Stem, Association Française contre les Myopathies (AFM), Institut National de la Santé et de la Recherche Médicale (INSERM), ²U861, I-Stem, Association Française contre les Myopathies (AFM), Université Evry Val-d'Essonne (UEVE), ³I-Stem, Association Française contre les Myopathies (AFM), Centre pour L’Etude des Cellules Souches (CECS), ⁴Banque de tissus humain, Hôpital Saint Louis, Assistance Publique - Hôpitaux de Paris (AP-HP), ⁵Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

We describe a method to engineer a retinal tissue composed of retinal pigment epithelial cells derived from human pluripotent stem cells cultured on top of human amniotic membranes and its preparation for grafting in animal models.