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Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Defined Xeno-free and Feeder-free Culture Conditions for the Generation of Human iPSC-derived Retinal Cell Models
Amélie Slembrouck-Brec *1, Céline Nanteau *1, José-Alain Sahel 1, Olivier Goureau 1, Sacha Reichman 1
1Institut de la Vision, Sorbonne Université, INSERM, CNRS, F-75012 Paris, France

The production of specialized retinal cells from pluripotent stem cells is a turning point in the development of stem cell-based therapy for retinal diseases. The present paper describes a simple method for an efficient generation of retinal organoids and retinal pigmented epithelium for basic, translational, and clinical research.

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Developmental Biology

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells
Karim Ben M'Barek 1,2,3, Walter Habeler 1,2,3, Alexandra Plancheron 1,2,3, Mohamed Jarraya 4, Olivier Goureau 5, Christelle Monville 1,2
1U861, I-Stem, Association Française contre les Myopathies (AFM), Institut National de la Santé et de la Recherche Médicale (INSERM), 2U861, I-Stem, Association Française contre les Myopathies (AFM), Université Evry Val-d'Essonne (UEVE), 3I-Stem, Association Française contre les Myopathies (AFM), Centre pour L’Etude des Cellules Souches (CECS), 4Banque de tissus humain, Hôpital Saint Louis, Assistance Publique - Hôpitaux de Paris (AP-HP), 5Sorbonne Université, INSERM, CNRS, Institut de la Vision, F-75012

We describe a method to engineer a retinal tissue composed of retinal pigment epithelial cells derived from human pluripotent stem cells cultured on top of human amniotic membranes and its preparation for grafting in animal models.

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