Wrocław University of Science and Technology

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Seongjin Park¹, Jiacheng Zhang², Matthew A. Reyer², Joanna Zareba^1,3, Andrew A. Troy⁴, Jingyi Fei^1,2

¹Department of Biochemistry and Molecular Biology, University of Chicago, ²The Institute for Biophysical Dynamics, University of Chicago, ³Faculty of Chemistry, Wrocław University of Science and Technology, ⁴Nikon Instruments Inc.

Here we present a practical guide of building an integrated microscopy system, which merges conventional epi-fluorescent imaging, single-molecule detection-based super-resolution imaging, and multi-color single-molecule detection, including single-molecule fluorescence resonance energy transfer imaging, into one set-up in a cost-efficient way.

Chemistry

Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization

Maciej Lipok¹, Patryk Obstarczyk¹, Joanna Olesiak-Bańska¹

¹Institute of Advanced Materials, Wrocław University of Science and Technology

This paper describes how polarization-sensitive two-photon microscopy could be applied to characterize the local organization within label-free amyloid superstructures-spherulites. It also describes how to prepare and measure the sample, assemble the required setup, and analyze the data to obtain information about the local organization of amyloid fibrils.