Wrocław University of Science and Technology
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Conducting Multiple Imaging Modes with One Fluorescence Microscope
Seongjin Park 1, Jiacheng Zhang 2, Matthew A. Reyer 2, Joanna Zareba 1,3, Andrew A. Troy 4, Jingyi Fei 1,2
1Department of Biochemistry and Molecular Biology, University of Chicago, 2The Institute for Biophysical Dynamics, University of Chicago, 3Faculty of Chemistry, Wrocław University of Science and Technology, 4Nikon Instruments Inc.
Here we present a practical guide of building an integrated microscopy system, which merges conventional epi-fluorescent imaging, single-molecule detection-based super-resolution imaging, and multi-color single-molecule detection, including single-molecule fluorescence resonance energy transfer imaging, into one set-up in a cost-efficient way.
Chemistry
Polarization-Sensitive Two-Photon Microscopy for a Label-Free Amyloid Structural Characterization
Maciej Lipok 1, Patryk Obstarczyk 1, Joanna Olesiak-Bańska 1
1Institute of Advanced Materials, Wrocław University of Science and Technology
This paper describes how polarization-sensitive two-photon microscopy could be applied to characterize the local organization within label-free amyloid superstructures-spherulites. It also describes how to prepare and measure the sample, assemble the required setup, and analyze the data to obtain information about the local organization of amyloid fibrils.
