Technical University of Graz

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Jasmin Elgin Fischer¹, Anna-Maria Hatzl², Astrid Weninger², Christian Schmid², Anton Glieder^1,2

¹bisy e.U., ²Institute of Molecular Biotechnology, Technical University of Graz

This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters P_DCand P_DF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.

JoVE Core

Fabrication of a Low-Cost, Fiber-Coupled, and Air-Spaced Fabry-Pérot Etalon

Manuel Tanzer¹, Benjamin Lang¹, Alexander Bergmann¹

¹Institute of Electrical Measurement and Sensor Systems, Technical University of Graz

This protocol describes the construction of a low-cost, discrete, fiber-coupled, and air-spaced Fabry-Perot etalon with various applications, such as in trace-gas spectroscopy. The fabrication is possible in any facility with standard optical laboratory equipment available.