Technical University of Graz
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
Jasmin Elgin Fischer 1, Anna-Maria Hatzl 2, Astrid Weninger 2, Christian Schmid 2, Anton Glieder 1,2
1bisy e.U., 2Institute of Molecular Biotechnology, Technical University of Graz
This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii), employing the carbon source regulated promoters PDCand PDF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied.
JoVE Core
Fabrication of a Low-Cost, Fiber-Coupled, and Air-Spaced Fabry-Pérot Etalon
Manuel Tanzer 1, Benjamin Lang 1, Alexander Bergmann 1
1Institute of Electrical Measurement and Sensor Systems, Technical University of Graz
This protocol describes the construction of a low-cost, discrete, fiber-coupled, and air-spaced Fabry-Perot etalon with various applications, such as in trace-gas spectroscopy. The fabrication is possible in any facility with standard optical laboratory equipment available.
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