Affiliated Hospital of Nanjing University of Chinese Medicine
2 ARTICLES PUBLISHED IN JoVE
Biology
Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
Lining Wang 1,2, Suyang Zheng 1,2, Yang Guo 1,2, Yalan Pan 1,2, Jie Sun 1,2, Weimin Xu 1,2, Jinlan Lu 3, Weidong Li 3, Yong Ma 1,2,4
1Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, 2TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory, Nanjing University of Chinese Medicine, 3Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, 4Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine
Osteoclasts are tissue-specific macrophage polykaryons derived from the monocyte-macrophage lineage of hematopoietic stem cells. This protocol describes how to isolate bone marrow cells so that large quantities of osteoclasts are obtained while reducing the risk of accidents found in traditional methods.
Medicine
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Hua-Qin Tong *1,2,3, Man-Lu Fan *3, Tong Sun 3, Hao-Wen Zhang 4, Jie Han 5, Meng-Xi Wang 3, Jian-Dong Chen 5, Wei-Xin Sun 6, Xiao-Hu Chen 1,2, Mian-Hua Wu 7
1Department of Cardiology, Affiliated Hospital of Nanjing University of Chinese Medicine, 2Department of Cardiology, Jiangsu Province Hospital of Chinese Medicine, 3First College of Clinical Medicine, Biological Technology Center for Innovation in Chinese Medicine, Nanjing University of Chinese Medicine, 4School of Health Preservation and Rehabilitation, Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, 5Department of Cardiology, Jiangsu Provincial Hospital of Chinese Medicine, 6Department of Cardiology, Yancheng TCM Hospital Affiliated to Nanjing University of Chinese Medicine, 7Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine
Myocardial ischemia-reperfusion model of rat heart is improved by using a self-made retractor, polyvinyl chloride tube, and a unique knotting method. Electrocardiogram, triphenyltetrazolium chloride and histological staining, and percent survival analysis results showed that the improved model group has higher success and survival rates than the already existing model group.
