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Affiliated Hospital of Nanjing University of Chinese Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Lining Wang^1,2, Suyang Zheng^1,2, Yang Guo^1,2, Yalan Pan^1,2, Jie Sun^1,2, Weimin Xu^1,2, Jinlan Lu³, Weidong Li³, Yong Ma^1,2,4

¹Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, ²TCM Nursing Intervention Laboratory of Chronic Disease Key Laboratory, Nanjing University of Chinese Medicine, ³Jiangsu Key Laboratory of Chinese Medicine Processing, Engineering Center of State Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, ⁴Department of Traumatology and Orthopedics, Affiliated Hospital of Nanjing University of Chinese Medicine

Osteoclasts are tissue-specific macrophage polykaryons derived from the monocyte-macrophage lineage of hematopoietic stem cells. This protocol describes how to isolate bone marrow cells so that large quantities of osteoclasts are obtained while reducing the risk of accidents found in traditional methods.

Medicine

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury

Hua-Qin Tong^*1,2,3, Man-Lu Fan^*3, Tong Sun³, Hao-Wen Zhang⁴, Jie Han⁵, Meng-Xi Wang³, Jian-Dong Chen⁵, Wei-Xin Sun⁶, Xiao-Hu Chen^1,2, Mian-Hua Wu⁷

¹Department of Cardiology, Affiliated Hospital of Nanjing University of Chinese Medicine, ²Department of Cardiology, Jiangsu Province Hospital of Chinese Medicine, ³First College of Clinical Medicine, Biological Technology Center for Innovation in Chinese Medicine, Nanjing University of Chinese Medicine, ⁴School of Health Preservation and Rehabilitation, Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, ⁵Department of Cardiology, Jiangsu Provincial Hospital of Chinese Medicine, ⁶Department of Cardiology, Yancheng TCM Hospital Affiliated to Nanjing University of Chinese Medicine, ⁷Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine (TCM) Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine

Myocardial ischemia-reperfusion model of rat heart is improved by using a self-made retractor, polyvinyl chloride tube, and a unique knotting method. Electrocardiogram, triphenyltetrazolium chloride and histological staining, and percent survival analysis results showed that the improved model group has higher success and survival rates than the already existing model group.