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Washington National Primate Research Center

2 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images
Karam Khateeb 1,2, Devon J. Griggs 2,3, Philip N. Sabes 4, Azadeh Yazdan-Shahmorad 1,2,3,4
1Departments of Bioengineering, University of Washington, 2Washington National Primate Research Center, 3Department of Electrical and Computer Engineering, University of Washington, 4Department of Physiology and Center for Integrative Neuroscience, University of California, San Francisco

Here, we demonstrate magnetic resonance (MR)-guided convection enhanced delivery (CED) of viral vectors into the cortex as an efficient and simplified approach for achieving optogenetic expression across large cortical areas in the macaque brain.

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Bioengineering

A MRI-Based Toolbox for Neurosurgical Planning in Nonhuman Primates
William K.S. Ojemann 1,2, Devon J. Griggs 2,3, Zachary Ip 1,2, Olivya Caballero 4, Hesamoddin Jahanian 5, Susana Martinez-Conde 4, Stephen Macknik 4, Azadeh Yazdan-Shahmorad 1,2,3
1Bioengineering Department, University of Washington, 2Washington National Primate Research Center, University of Washington, 3Electrical and Computer Engineering Department, University of Washington, 4Department of Ophthalmology, SUNY Downstate Health Sciences University, 5Radiology Department, University of Washington

The method outlined below aims to provide a comprehensive protocol for the preparation of nonhuman primate (NHP) neurosurgery using a novel combination of three-dimensional (3D) printing methods and MRI data extraction.

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