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Washington National Primate Research Center

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Convection Enhanced Delivery of Optogenetic Adeno-associated Viral Vector to the Cortex of Rhesus Macaque Under Guidance of Online MRI Images

Karam Khateeb^1,2, Devon J. Griggs^2,3, Philip N. Sabes⁴, Azadeh Yazdan-Shahmorad^1,2,3,4

¹Departments of Bioengineering, University of Washington, ²Washington National Primate Research Center, ³Department of Electrical and Computer Engineering, University of Washington, ⁴Department of Physiology and Center for Integrative Neuroscience, University of California, San Francisco

Here, we demonstrate magnetic resonance (MR)-guided convection enhanced delivery (CED) of viral vectors into the cortex as an efficient and simplified approach for achieving optogenetic expression across large cortical areas in the macaque brain.

Bioengineering

A MRI-Based Toolbox for Neurosurgical Planning in Nonhuman Primates

William K.S. Ojemann^1,2, Devon J. Griggs^2,3, Zachary Ip^1,2, Olivya Caballero⁴, Hesamoddin Jahanian⁵, Susana Martinez-Conde⁴, Stephen Macknik⁴, Azadeh Yazdan-Shahmorad^1,2,3

¹Bioengineering Department, University of Washington, ²Washington National Primate Research Center, University of Washington, ³Electrical and Computer Engineering Department, University of Washington, ⁴Department of Ophthalmology, SUNY Downstate Health Sciences University, ⁵Radiology Department, University of Washington

The method outlined below aims to provide a comprehensive protocol for the preparation of nonhuman primate (NHP) neurosurgery using a novel combination of three-dimensional (3D) printing methods and MRI data extraction.