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Netherlands Cancer Institute

2 ARTICLES PUBLISHED IN JoVE

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Cancer Research

A Practical Guide for the Production and PET/CT Imaging of 68Ga-DOTATATE for Neuroendocrine Tumors in Daily Clinical Practice
Else A. Aalbersberg 1, Martine M. Geluk-Jonker 1, Thirumaraichelvi Young-Mylvaganan 1, Linda J. de Wit-van der Veen 1, Marcel P. M. Stokkel 1
1Department of Nuclear Medicine, Netherlands Cancer Institute

Well-differentiated neuroendocrine tumors overexpress somatostatin receptors which can be utilized for diagnostic imaging with the radiolabeled somatostatin analog 68Ga-DOTATATE. This protocol details the radiolabeling of 68Ga-DOTATATE, quality control, patient preparation, and subsequent PET/CT imaging. Radiation safety and time constrictions due to the short half-life of 68Ga are taken into account.

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Biochemistry

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
Jitske Bak 1, Lisa Landskron 1, Thijn R. Brummelkamp 1, Anastassis Perrakis 1
1Oncode Institute and Division of Biochemistry, Netherlands Cancer Institute

Here, we describe a protocol to extract endogenous tubulin from mammalian cells, which can lack or contain specific microtubule-modifying enzymes, to obtain microtubules enriched for a specific modification. We then describe how the extracted microtubules can be decorated with purified microtubule-binding proteins to prepare grids for cryo-electron microscopy.

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