National Human Genetic Resources Sharing Service Platform (2005DKA21300)

3 ARTICLES PUBLISHED IN JoVE

Environment

Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

Huan Chen^*1,2, Ian Arthur Palmer^*1, Jian Chen^1,2, Ming Chang^1,2, Stephen L. Thompson³, Fengquan Liu², Zheng Qing Fu¹

¹Department of Biological Sciences, University of South Carolina, ²Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, ³Department of Instructions and Teacher Education, University of South Carolina

Citrus Greening is a particularly destructive disease affecting citrus crops globally. Presented here is a simple method using PCR and genomic DNA extraction of citrus leaf tissue for the accurate and precise identification of the citrus greening pathogen, Candidatus liberibacter spp.

Cancer Research

Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment

Lei Wang^*1, Huan Chen^*2,3, Fei Fei^*1, Xianfeng He^2,3, Shaoyang Sun¹, Kunpeng Lv¹, Bo Yu^2,3, Jiang Long^2,4,5,6, Xu Wang¹

¹Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences, Fudan University, ²National Human Genetic Resources Sharing Service Platform (2005DKA21300), ³Fudan University Shanghai Cancer Center, ⁴Department of Pancreatic Surgery, Fudan University Shanghai Cancer Center, ⁵Department of Oncology, Shanghai Medical College, Fudan University, ⁶Pancreatic Cancer Institute, Fudan University

This protocol describes optimization procedures in a virus-based dual fluorescence-labeled tumor xenograft model using larval zebrafish as hosts. This heterogeneous xenograft model mimics the tissue composition of pancreatic cancer microenvironment in vivo and serves as a more precise tool for assessing drug responses in personalized zPDX (zebrafish patient-derived xenograft) models.

Medicine

A Training and Testing System for Performing Vascular Reconstruction In Vitro

Yue Wang^1,2, Lijun Mu³, Wei Zhang^1,2, Huan Chen^1,2, Qingshan Li^1,2, Aihua Shi¹, Bo Tang², Xiaogang Zhang^1,2, Dinghui Dong^1,2, Yi Lv^1,2

¹National Local Joint Engineering Research Center for Precision Surgery and Regenerative Medicine, First Affiliated Hospital of Xi'an Jiaotong University, ²Department of Hepatobiliary Surgery, First Affiliated Hospital of Xi'an Jiaotong University, ³Department of General Surgery, First Affiliated Hospital of Xi'an Jiaotong University

Here we present a training and testing system where a trainee can complete manual vascular reconstruction in vitro individually using a magnetic anchoring technique. The system can also be used to test the quality of reconstruction.