Institut Pasteur de Guinée, Conakry

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

¹Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus, Institut Pasteur, ²Institut Pasteur de Guinée, Conakry

Here we describe an indirect ELISA sandwich immunocapture to determine the immunogenic glycoprotein contents in rabies vaccines. This test uses a neutralizing Monoclonal Antibody (mAb-D1) recognizing glycoprotein trimers. It is an alternative to the in vivo NIH test to follow the consistency of vaccine potency during production.