Vienna Biocenter (VBC)
3 ARTICLES PUBLISHED IN JoVE
Neuroscience
In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
Wilson Pak-Kin Lou 1, Avni Baser 1, Stefan Klußmann 1, Ana Martin-Villalba 1
1Department of Molecular Neurobiology, German Cancer Research Center (DKFZ)
This protocol illustrates essential modifications of polyribosome fractionation in order to study the translatome of in vivo CNS samples. It allows global assessment of translation and transcription regulation through the isolation and comparison of total RNA to ribosome bound RNA fractions.
Genetics
Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes
Wilson Pak-Kin Lou *1,2, Liqun Wang *3, Cheng Long 1, Lei Liu 3, Ji-Feng Fei 3
1School of Life Sciences, South China Normal University, 2The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Institute for Brain Research and Rehabilitation (IBRR), South China Normal University
Presented here is a protocol to perform time- and space-restricted gene knock-out in axolotl spinal cords by injecting CAS9-gRNA complex into the spinal cord central canal followed by electroporation.
Developmental Biology
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
Harunobu Kagawa *1, Alok Javali *1, Heidar Heidari Khoei *1, Theresa Maria Sommer 1,3, Giovanni Sestini 1,3, Maria Novatchkova 2, Yvonne Scholte op Reimer 1, Nicolas Rivron 1
1Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), 2Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), 3Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna
A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.
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