Vienna Biocenter (VBC)

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

Wilson Pak-Kin Lou¹, Avni Baser¹, Stefan Klußmann¹, Ana Martin-Villalba¹

¹Department of Molecular Neurobiology, German Cancer Research Center (DKFZ)

This protocol illustrates essential modifications of polyribosome fractionation in order to study the translatome of in vivo CNS samples. It allows global assessment of translation and transcription regulation through the isolation and comparison of total RNA to ribosome bound RNA fractions.

Genetics

Direct Gene Knock-out of Axolotl Spinal Cord Neural Stem Cells via Electroporation of CAS9 Protein-gRNA Complexes

Wilson Pak-Kin Lou^*1,2, Liqun Wang^*3, Cheng Long¹, Lei Liu³, Ji-Feng Fei³

¹School of Life Sciences, South China Normal University, ²The Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), ³Institute for Brain Research and Rehabilitation (IBRR), South China Normal University

Presented here is a protocol to perform time- and space-restricted gene knock-out in axolotl spinal cords by injecting CAS9-gRNA complex into the spinal cord central canal followed by electroporation.

Developmental Biology

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Harunobu Kagawa^*1, Alok Javali^*1, Heidar Heidari Khoei^*1, Theresa Maria Sommer^1,3, Giovanni Sestini^1,3, Maria Novatchkova², Yvonne Scholte op Reimer¹, Nicolas Rivron¹

¹Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna Biocenter (VBC), ²Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), ³Vienna BioCenter PhD Program, Doctoral School of the University of Vienna and Medical University of Vienna

A protocol outlining the formation of human blastoids that efficiently, timely, and sequentially generate blastocyst-like cells.