Purification of High Yield Extracellular Vesicle Preparations Away from VirusCatherine DeMarino *1, Robert A. Barclay *1, Michelle L. Pleet 1, Daniel O. Pinto 1, Heather Branscome 1,2, Siddhartha Paul 3, Benjamin Lepene 4, Nazira El-Hage 5, Fatah Kashanchi 1
1Laboratory of Molecular Virology, School of Systems Biology, George Mason University, 2American Type Culture Collection (ATCC), 3ATCC Cell Systems, 4Ceres Nanosciences, Inc, 5Department of Immunology and Nano-medicine, Herbert Wertheim College of Medicine, Florida International University
This protocol isolates extracellular vesicles (EVs) away from virions with high efficiency and yield by incorporating EV precipitation, density gradient ultracentrifugation, and particle capture to allow for a streamlined workflow and a reduction of starting volume requirements, resulting in reproducible preparations for use in all EV research.
