University of Colorado - Anschutz Medical Campus

1 ARTICLES PUBLISHED IN JoVE

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Cancer Research

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
Michael Seager 1, Dara L. Aisner 1, Kurtis D. Davies 1
1Department of Pathology, University of Colorado - Anschutz Medical Campus

This article details the use of an anchored multiplex polymerase chain reaction-based library preparation kit followed by next-generation sequencing to assess for oncogenic gene fusions in clinical solid tumor samples. Both wet-bench and data analysis steps are described.

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