Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation SequencingMichael Seager 1, Dara L. Aisner 1, Kurtis D. Davies 1
1Department of Pathology, University of Colorado - Anschutz Medical Campus
This article details the use of an anchored multiplex polymerase chain reaction-based library preparation kit followed by next-generation sequencing to assess for oncogenic gene fusions in clinical solid tumor samples. Both wet-bench and data analysis steps are described.
