Federal Rural University of Rio de Janeiro

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies

Jéssica Fiorotti¹, Patrícia S. Gôlo^1,2, Allan Felipe Marciano¹, Mariana G. Camargo¹, Isabele C. Angelo^1,3, Vânia R. E. P. Bittencourt^1,2

¹Post-Graduate Program in Veterinary Sciences, Federal Rural University of Rio de Janeiro, ²Department of Animal Parasitology, Federal Rural University of Rio de Janeiro, ³Department of Epidemiology and Public Health, Federal Rural University of Rio de Janeiro

Analysis of tick hemolymph represents an important source of information on how some pathogens cause disease and how ticks immunologically respond to this infection. The present study demonstrates how to inoculate fungal propagules and collect hemolymph from Rhipicephalus microplus engorged females.

Biology

Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples

Thais A. Correa¹, Fernanda S. Santos², Mariana G. Camargo³, Simone Quinelato², Vânia R. E. P. Bittencourt^1,3, Patricia S. Golo^1,3

¹Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, ²Laboratório de Taxonomia Bioquímica e Bioprospecção de Fungos/Coleção de Cultura de Fungos Filamentosos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), ³Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro

Entomopathogenic fungal colonies are isolated from tropical soil samples using Tenebrio bait, Galleria bait, as well as selective artificial medium, i.e., potato dextrose agar enriched with yeast extract supplemented with chloramphenicol, thiabendazole, and cycloheximide (CTC medium).