Federal Rural University of Rio de Janeiro
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Disclosing Hemolymph Collection and Inoculation of Metarhizium Blastospores into Rhipicephalus Microplus Ticks Towards Invertebrate Pathology Studies
Jéssica Fiorotti 1, Patrícia S. Gôlo 1,2, Allan Felipe Marciano 1, Mariana G. Camargo 1, Isabele C. Angelo 1,3, Vânia R. E. P. Bittencourt 1,2
1Post-Graduate Program in Veterinary Sciences, Federal Rural University of Rio de Janeiro, 2Department of Animal Parasitology, Federal Rural University of Rio de Janeiro, 3Department of Epidemiology and Public Health, Federal Rural University of Rio de Janeiro
Analysis of tick hemolymph represents an important source of information on how some pathogens cause disease and how ticks immunologically respond to this infection. The present study demonstrates how to inoculate fungal propagules and collect hemolymph from Rhipicephalus microplus engorged females.
Biology
Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples
Thais A. Correa 1, Fernanda S. Santos 2, Mariana G. Camargo 3, Simone Quinelato 2, Vânia R. E. P. Bittencourt 1,3, Patricia S. Golo 1,3
1Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, 2Laboratório de Taxonomia Bioquímica e Bioprospecção de Fungos/Coleção de Cultura de Fungos Filamentosos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), 3Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro
Entomopathogenic fungal colonies are isolated from tropical soil samples using Tenebrio bait, Galleria bait, as well as selective artificial medium, i.e., potato dextrose agar enriched with yeast extract supplemented with chloramphenicol, thiabendazole, and cycloheximide (CTC medium).
