University of Vermont Larner College of Medicine
2 ARTICLES PUBLISHED IN JoVE
Biology
Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events
Kathryn V. Svec 1,2,3, Johnathan B Patterson 1,2,3, Nyla Naim 1,2,3, Alan K Howe 1,2,3
1Department of Pharmacology, University of Vermont Larner College of Medicine, 2University of Vermont Cancer Center, 3Department of Molecular Physiology and Biophysics, University of Vermont Larner College of Medicine
Mechanical forces are important for controlling cell migration. This protocol demonstrates the use of elastic hydrogels that can be deformed using a glass micropipette and a micromanipulator to stimulate cells with a local stiffness gradient to elicit changes in cell structure and migration.
Neuroscience
Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study
Amanda C. Rosehart 1, Abbie C. Johnson 2, Fabrice Dabertrand 1,3
1Department of Anesthesiology, University of Colorado Anschutz Medical Campus, 2Department of Neurological Sciences, University of Vermont Larner College of Medicine, 3Department of Pharmacology, University of Colorado Anschutz Medical Campus
The present manuscript details how to isolate hippocampal arterioles and capillaries from the mouse brain and how to pressurize them for pressure myography, immunofluorescence, biochemistry, and molecular studies.
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