University of Vermont Larner College of Medicine

2 ARTICLES PUBLISHED IN JoVE

Biology

Single Cell Durotaxis Assay for Assessing Mechanical Control of Cellular Movement and Related Signaling Events

Kathryn V. Svec^1,2,3, Johnathan B Patterson^1,2,3, Nyla Naim^1,2,3, Alan K Howe^1,2,3

¹Department of Pharmacology, University of Vermont Larner College of Medicine, ²University of Vermont Cancer Center, ³Department of Molecular Physiology and Biophysics, University of Vermont Larner College of Medicine

Mechanical forces are important for controlling cell migration. This protocol demonstrates the use of elastic hydrogels that can be deformed using a glass micropipette and a micromanipulator to stimulate cells with a local stiffness gradient to elicit changes in cell structure and migration.

Neuroscience

Ex Vivo Pressurized Hippocampal Capillary-Parenchymal Arteriole Preparation for Functional Study

Amanda C. Rosehart¹, Abbie C. Johnson², Fabrice Dabertrand^1,3

¹Department of Anesthesiology, University of Colorado Anschutz Medical Campus, ²Department of Neurological Sciences, University of Vermont Larner College of Medicine, ³Department of Pharmacology, University of Colorado Anschutz Medical Campus

The present manuscript details how to isolate hippocampal arterioles and capillaries from the mouse brain and how to pressurize them for pressure myography, immunofluorescence, biochemistry, and molecular studies.