Universität Innsbruck
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions
Andreas O. Wagner *1, Rudolf Markt *1, Mira Mutschlechner *1, Nina Lackner *1, Eva M. Prem *1, Nadine Praeg 1, Paul Illmer 1
1Department of Microbiology, Universität Innsbruck
As obligate anaerobic organisms are unable to grow upon oxygen exposure, the use of anaerobic culturing techniques is indispensable. Here, we demonstrate a simple and effective method to cultivate a mixed culture derived from a biogas plant from media preparation to gas and volatile fatty acid quantification.
Environment
Extraction of Cofactor F420 for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples
Rudolf Markt 1, Mathias Wunderer 1, Eva Maria Prem 1, Mira Mutschlechner 1, Nina Lackner 2, Andreas Otto Wagner 1
1Department of Microbiology, Universität Innsbruck, 2Department of Hygiene and Medical Microbiology, Medical University of Innsbruck
A method for the extraction of cofactor F420 from pure cultures was optimized for the liquid chromatographic separation and analysis of F420 tail length in pure culture and environmental samples.
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