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Universität Innsbruck

2 ARTICLES PUBLISHED IN JoVE

Biochemistry

Medium Preparation for the Cultivation of Microorganisms under Strictly Anaerobic/Anoxic Conditions

Andreas O. Wagner^*1, Rudolf Markt^*1, Mira Mutschlechner^*1, Nina Lackner^*1, Eva M. Prem^*1, Nadine Praeg¹, Paul Illmer¹

¹Department of Microbiology, Universität Innsbruck

As obligate anaerobic organisms are unable to grow upon oxygen exposure, the use of anaerobic culturing techniques is indispensable. Here, we demonstrate a simple and effective method to cultivate a mixed culture derived from a biogas plant from media preparation to gas and volatile fatty acid quantification.

Environment

Extraction of Cofactor F₄₂₀ for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

Rudolf Markt¹, Mathias Wunderer¹, Eva Maria Prem¹, Mira Mutschlechner¹, Nina Lackner², Andreas Otto Wagner¹

¹Department of Microbiology, Universität Innsbruck, ²Department of Hygiene and Medical Microbiology, Medical University of Innsbruck

A method for the extraction of cofactor F₄₂₀ from pure cultures was optimized for the liquid chromatographic separation and analysis of F₄₂₀ tail length in pure culture and environmental samples.