Research Fellow of Japan Society for the Promotion of Science

1 ARTICLES PUBLISHED IN JoVE

Medicine

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Kazuma Sekiba^1,2, Motoyuki Otsuka¹, Kazuhiko Koike¹

¹Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, ²Research Fellow of Japan Society for the Promotion of Science

Here, we present a method for screening anti-hepatitis B viral agents that inhibit the HBx-DDB1 interaction using a split luciferase assay system. This system allows easy detection of protein-protein interactions and is suitable for identifying inhibitors of such interactions.