Friedrich-Alexander University Erlangen-Nuernberg

1 ARTICLES PUBLISHED IN JoVE

JoVE Core

Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays

Judith A. Stolwijk¹, Anne-Kathrin Mildner¹, Christian Kade¹, Michael Skiba¹, Guenther Bernhardt², Armin Buschauer², Harald Huebner³, Peter Gmeiner³, Joachim Wegener^1,4

¹Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, ²Institute of Pharmacy, University of Regensburg, ³Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, ⁴Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.