Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR AssaysJudith A. Stolwijk 1, Anne-Kathrin Mildner 1, Christian Kade 1, Michael Skiba 1, Guenther Bernhardt 2, Armin Buschauer 2, Harald Huebner 3, Peter Gmeiner 3, Joachim Wegener 1,4
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT
This protocol demonstrates real-time recording of full dose-response relationships for agonist-induced GPCR activation from a single cell layer grown on a single microelectrode using label-free impedance measurements. The new dosing scheme significantly increases throughput without loss in time resolution.
