Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM

1 ARTICLES PUBLISHED IN JoVE

Genetics

3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary Cells

Federica Marasca¹, Alice Cortesi¹, Lara Manganaro¹, Beatrice Bodega¹

¹Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM

3D multicolor DNA FISH represents a tool to visualize multiple genomic loci within 3D preserved nuclei, unambiguously defining their reciprocal interactions and localization within the nuclear space at a single cell level. Here, a step by step protocol is described for a wide spectrum of human primary cells.