3D Multicolor DNA FISH Tool to Study Nuclear Architecture in Human Primary CellsFederica Marasca 1, Alice Cortesi 1, Lara Manganaro 1, Beatrice Bodega 1
1Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM
3D multicolor DNA FISH represents a tool to visualize multiple genomic loci within 3D preserved nuclei, unambiguously defining their reciprocal interactions and localization within the nuclear space at a single cell level. Here, a step by step protocol is described for a wide spectrum of human primary cells.
