An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding PerformanceLuz DC. Martínez-Sánchez *1, Rashmita Pradhan *1, Phuong A. Ngo 1, Lena Erkert 1, Lukas S. Becker 1, Alastair J. Watson 2, Imke Atreya *1, Markus F. Neurath *1, Rocío López-Posadas *1
1Medical Clinic 1, University of Erlangen-Nuremberg, University Hospital of Erlangen, 2Norwich Medical School, University of East Anglia, Norwich Research Park
Taking advantage of intravital microscopy, the method presented here enables real-time visualization of intestinal epithelial cell shedding in living animals. Therefore, topically stained intestinal mucosa (acriflavine and rhodamineB-dextran) of anesthetized mice is imaged up to single-cell resolution using confocal microscopy.
