Braunschweig Integrated Centre of Systems Biology
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
Hilger Jagau 1,2, Ina-Kristin Behrens 1,3, Michael Steinert 1,4, Simone Bergmann 1
1Institut für Mikrobiologie, Technische Universität Braunschweig, 2Devision of Infection Medicine, Department of Clinical Science, Lund University, 3Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, 4Department of Molecular Infection Biology, Helmholtz Center for Infection Research
This study describes the microscopic monitoring of pneumococcus adherence to von Willebrand factor strings produced on the surface of differentiated human primary endothelial cells under shear stress in defined flow conditions. This protocol can be extended to detailed visualization of specific cell structures and quantification of bacteria by applying differential immunostaining procedures.
Developmental Biology
Development of a Larval Zebrafish Infection Model for Clostridioides difficile
Junkai Li 1, Can M. Ünal 2, Kazuhiko Namikawa 1, Michael Steinert 3,4,5, Reinhard W. Köster 1
1Division of Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, 2Department of Molecular Biotechnology, Turkish-German University, 3Institut für Mikrobiologie, Technische Universität Braunschweig, 4Braunschweig Integrated Centre of Systems Biology, 5Helmholtz Centre for Infection Research
Presented here is a safe and effective method to infect zebrafish larvae with fluorescently labeled anaerobic C. difficile by microinjection and noninvasive microgavage.
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