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Braunschweig Integrated Centre of Systems Biology

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow

Hilger Jagau^1,2, Ina-Kristin Behrens^1,3, Michael Steinert^1,4, Simone Bergmann¹

¹Institut für Mikrobiologie, Technische Universität Braunschweig, ²Devision of Infection Medicine, Department of Clinical Science, Lund University, ³Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Ludwig Maximilians University, ⁴Department of Molecular Infection Biology, Helmholtz Center for Infection Research

This study describes the microscopic monitoring of pneumococcus adherence to von Willebrand factor strings produced on the surface of differentiated human primary endothelial cells under shear stress in defined flow conditions. This protocol can be extended to detailed visualization of specific cell structures and quantification of bacteria by applying differential immunostaining procedures.

Developmental Biology

Development of a Larval Zebrafish Infection Model for Clostridioides difficile

Junkai Li¹, Can M. Ünal², Kazuhiko Namikawa¹, Michael Steinert^3,4,5, Reinhard W. Köster¹

¹Division of Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, ²Department of Molecular Biotechnology, Turkish-German University, ³Institut für Mikrobiologie, Technische Universität Braunschweig, ⁴Braunschweig Integrated Centre of Systems Biology, ⁵Helmholtz Centre for Infection Research

Presented here is a safe and effective method to infect zebrafish larvae with fluorescently labeled anaerobic C. difficile by microinjection and noninvasive microgavage.