GH Pitié Salpêtrière
2 ARTICLES PUBLISHED IN JoVE
Neuroscience
Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
Melina Thetiot 1, Rémi Ronzano 1, Marie-Stéphane Aigrot 1, Catherine Lubetzki 1, Anne Desmazières 1
1Sorbonne Université, Inserm, CNRS, Institut du Cerveau et de la Moelle épinière, ICM-GH Pitié-Salpétrière
Here we present a method to prepare organotypic slice cultures from mouse cerebellum and myelin sheath staining by immunohistochemistry suitable for investigating mechanisms of myelination and remyelination in the central nervous system.
Neuroscience
Generation of Oligodendrocytes and Oligodendrocyte-Conditioned Medium for Co-Culture Experiments
Elisa Mazuir 1, Anne-Laure Dubessy 1,2, Loane Wallon 1,3, Marie-Stéphane Aigrot 1, Catherine Lubetzki 1,2, Nathalie Sol-Foulon 1
1Sorbonne Université, Inserm, CNRS, UMR7225, Institut du Cerveau et de la Moelle épinière, ICM, 2Assistance Publique-Hôpitaux de Paris, GH Pitié Salpêtrière, 3Ecole Supérieure des Techniques de Biologie Appliquée
Herein, we display an efficient method for the purification of oligodendrocytes and production of oligodendrocyte-conditioned medium that can be used for co-culture experiments.
