University of Neuchâtel
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
Martha Thellmann 1, Tonni Grube Andersen 2, Joop EM Vermeer 1,3
1Department of Plant and Microbial Biology, University of Zurich, 2Biophore - UNIL, 3Laboratory of Cell and Molecular Biology, Institute of Biology, University of Neuchâtel
Translating ribosome affinity purification (TRAP) offers the possibility to dissect developmental programs with minimal processing of organs and tissues. The protocol yields high-quality RNA from cells targeted with a green fluorescent protein (GFP)-labeled ribosomal subunit. Downstream analysis tools, such as qRT-PCR or RNA-seq, reveal tissue and cell type-specific expression profiles.
Biology
Investigating Bacterial-Fungal Interactions using Fungal Highway Columns in Diverse Environments and Substrates
Julia M. Kelliher 1, Leah Y. D. Johnson 1, Aaron J. Robinson 1, Ilona Palmieri 2, Buck T. Hanson 1, Reid Longley 1, La Verne Gallegos-Graves 1, Kaelan Prime 1, Guillaume Cailleau 2, Saskia Bindschedler 2, Patrick S. G. Chain 1, Pilar Junier 2
1Bioscience Division, Los Alamos National Laboratory, 2University of Neuchâtel
This protocol provides detailed instructions on how to construct, sterilize, assemble, utilize, and reuse the fungal highway columns to enrich bacterial-fungal pairs interacting through fungal highways from diverse environmental substrates.
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