Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific ScaleMartha Thellmann 1, Tonni Grube Andersen 2, Joop EM Vermeer 1,3
1Department of Plant and Microbial Biology, University of Zurich, 2Biophore - UNIL, 3Laboratory of Cell and Molecular Biology, Institute of Biology, University of Neuchâtel
Translating ribosome affinity purification (TRAP) offers the possibility to dissect developmental programs with minimal processing of organs and tissues. The protocol yields high-quality RNA from cells targeted with a green fluorescent protein (GFP)-labeled ribosomal subunit. Downstream analysis tools, such as qRT-PCR or RNA-seq, reveal tissue and cell type-specific expression profiles.
