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Tel Aviv University Center for Light-Matter Interaction

2 ARTICLES PUBLISHED IN JoVE

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Biology

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation
Itamar Cohen 1, Yifat Geffen 1, Guy Ravid 1, Tommer Ravid 1
1Department of Biological Chemistry, The Hebrew University of Jerusalem

Here we describe a robust biological assay for quantifying the relative rate of proteolysis by the ubiquitin-proteasome system. The assay readout is yeast growth rate in liquid culture, which is dependent on the cellular levels of a reporter protein comprising a degradation signal fused to an essential metabolic marker.

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Engineering

Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments
Yonatan Gershuni 1,2, Michal Elkind 1,2, Dolev Roitman 1,2, Itamar Cohen 1,2, Aviad Tsabary 1,2, Deep Sarkar 1,2, Ishay Pomerantz 1,2
1The School of Physics and Astronomy, Tel Aviv University, 2Tel Aviv University Center for Light-Matter Interaction

A protocol is presented for automated irradiation of thin gold foils with high intensity laser pulses. The protocol includes a step-by-step description of the micromachining target fabrication process and a detailed guide for how targets are brought to the laser's focus at a rate of 0.2 Hz.

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