S'identifier

Tel Aviv University Center for Light-Matter Interaction

2 ARTICLES PUBLISHED IN JoVE

Biology

Reporter-based Growth Assay for Systematic Analysis of Protein Degradation

Itamar Cohen¹, Yifat Geffen¹, Guy Ravid¹, Tommer Ravid¹

¹Department of Biological Chemistry, The Hebrew University of Jerusalem

Here we describe a robust biological assay for quantifying the relative rate of proteolysis by the ubiquitin-proteasome system. The assay readout is yeast growth rate in liquid culture, which is dependent on the cellular levels of a reporter protein comprising a degradation signal fused to an essential metabolic marker.

Engineering

Automated Delivery of Microfabricated Targets for Intense Laser Irradiation Experiments

Yonatan Gershuni^1,2, Michal Elkind^1,2, Dolev Roitman^1,2, Itamar Cohen^1,2, Aviad Tsabary^1,2, Deep Sarkar^1,2, Ishay Pomerantz^1,2

¹The School of Physics and Astronomy, Tel Aviv University, ²Tel Aviv University Center for Light-Matter Interaction

A protocol is presented for automated irradiation of thin gold foils with high intensity laser pulses. The protocol includes a step-by-step description of the micromachining target fabrication process and a detailed guide for how targets are brought to the laser's focus at a rate of 0.2 Hz.