University of Aveiro

4 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Organ Culture and Whole Mount Immunofluorescence Staining of Mouse Wolffian Ducts

¹Gynaecology Oncology Group, School of Biomedical Sciences and Pharmacy, University of Newcastle

We present a method for isolation and culture of the mouse Wolffian duct (WD). We also demonstrate a detailed procedure for whole mount immunostaining of cultured/freshly isolated WDs with fluorescently tagged antibodies. Together, these techniques enable the study of WD development, coiling, and differentiation.

Engineering

Development of Efficient OLEDs from Solution Deposition

Manish Kumar^1,2, Luiz Pereira¹

¹Department of Physics and i3N, Institute for Nanostructures, Nanomodulation and Nanofabrication, University of Aveiro, ²CeNTI, Centre for Nanotechnologies and Smart Materials

Presented here is a protocol for the fabrication of efficient, simple, solution-deposited organic light-emitting diodes with low roll-off.

Neuroscience

Isolation and Culture of Chick Ciliary Ganglion Neurons

Filipa J. Costa^*1, Marta S. Dias^*1, Rui O. Costa², Joana R. Pedro², Ramiro D. Almeida^1,2

¹Institute of Biomedicine, Department of Medical Sciences - iBiMED, University of Aveiro, ²CNC - Center for Neuroscience and Cell Biology, University of Coimbra

Chick ciliary ganglia (CG) are part of the parasympathetic nervous system. Neuronal cultures of chick CG neurons were shown to be effective cell models in the study of nerve muscle interactions. We describe a detailed protocol for the dissection, dissociation and in vitro culture of CG neurons from chick embryos.

Neuroscience

Optimized Automated Analysis of Live Neuronal Mitochondria Homeostasis Modulation by Isoform-Specific Retinoic Acid Receptors

José J. M. Vitória¹, Vinícius de Paula¹, Odete A. B. da Cruz e Silva¹, Diogo Trigo¹

¹Neuroscience and Signalling Laboratory, Institute of Biomedicine (iBiMED), Department of Medical Sciences, University of Aveiro

The mitochondrial network is extremely complex, making it very challenging to analyze. A novel MATLAB tool analyzes live confocal imaged mitochondria in timelapse images but results in a large output volume requiring individual manual attention. To address this issue, a routine optimization was developed, allowing for speedy file analysis.