Leipzig University

3 ARTICLES PUBLISHED IN JoVE

Biology

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T

Remco van Schadewijk¹, Julia R. Krug^2,3,4, Andrew Webb⁵, Henk Van As^2,4, Aldrik H. Velders^3,4, Huub J. M. de Groot¹, A. Alia^1,6

¹Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science, Leiden University, ²Laboratory of Biophysics, Wageningen University & Research, ³Laboratory of BioNanoTechnology, Wageningen University & Research, ⁴MAGNEtic resonance Facility, Wageningen University & Research, ⁵C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre, Leiden University, ⁶Institute for Medical Physics and Biophysics, Leipzig University

A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.

Biochemistry

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Raphaël de Wijn^1,4, Kévin Rollet^1,2, Vincent Olieric³, Oliver Hennig², Nicola Thome¹, Camille Noûs¹, Caroline Paulus¹, Bernard Lorber¹, Heike Betat², Mario Mörl², Claude Sauter¹

¹Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, ²Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, ³Paul Scherrer Institute, Swiss Light Source, ⁴European XFEL GmbH

A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature.

Neuroscience

Neuronavigated Focalized Transcranial Direct Current Stimulation Administered During Functional Magnetic Resonance Imaging

Filip Niemann^*1, Alireza Shahbabaie^*1, Sven Paßmann¹, Steffen Riemann¹, Robert Malinowski¹, Harun Kocataş¹, Leonardo M. Caisachana Guevara^1,2, Mohamed Abdelmotaleb¹, Daria Antonenko¹, Felix Blankenburg^3,4, Rico Fischer², Gesa Hartwigsen^5,6, Shu-Chen Li^7,8, Michael A. Nitsche^9,10,11, Axel Thielscher^12,13, Dagmar Timmann¹⁴, Anna Fromm¹, Dayana Hayek¹, Ann-Kathrin Hubert¹, Andrew K. Martin^15,16, Alexander Hunold¹⁷, Agnes Flöel^*1,18, Marcus Meinzer^*1

¹Department of Neurology, University Medicine Greifswald, ²Department of Psychology, University Medicine Greifswald, ³Berlin School of Mind and Brain, Humboldt Universität zu Berlin, ⁴Neurocomputation and Neuroimaging Unit, Freie Universität Berlin, ⁵Wilhelm-Wundt-Institute for Psychology, Cognitive and Biological Psychology, Leipzig University, ⁶Lise Meitner Research Group Cognition and Plasticity, Max Planck Institute for Human Cognitive and Brain Sciences, ⁷Lifespan Developmental Neuroscience, Faculty of Psychology, Technische Universität Dresden, ⁸Centre for Tactile Internet with Human-in-the-Loop, Technische Universität Dresden, ⁹Department of Psychology and Neurosciences, Leibniz Research Centre for Working Environment and Human Factors, ¹⁰German Centre for Mental Health (DZPG), ¹¹Clinic of Psychiatry and Psychotherapy, Protestant Hospital of Bethel Foundation, University Hospital OWL, Bielefeld University, ¹²Danish Research Centre for Magnetic Resonance, Centre for Functional and Diagnostic Imaging and Research, Copenhagen University Hospital Amager and Hvidovre, ¹³Department of Health Technology, Technical University of Denmark, ¹⁴Department of Neurology and Center for Translational Neuro- and Behavioral Sciences (C-TNBS), Essen University Hospital, ¹⁵Department of Psychology, University of Kent, ¹⁶Kent Medway Medical School, ¹⁷Institute of Biomedical Engineering and Informatics, Technische Universität Ilmenau, ¹⁸German Center for Neurodegenerative Diseases (DZNE Site Greifswald)

This protocol describes the method of neuronavigated electrode placement for focal, transcranial direct current stimulation (tDCS) administered during functional magnetic resonance imaging (fMRI).