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Leipzig University

2 ARTICLES PUBLISHED IN JoVE

Biology

MRM Microcoil Performance Calibration and Usage Demonstrated on Medicago truncatula Roots at 22 T

Remco van Schadewijk¹, Julia R. Krug^2,3,4, Andrew Webb⁵, Henk Van As^2,4, Aldrik H. Velders^3,4, Huub J. M. de Groot¹, A. Alia^1,6

¹Solid-state NMR, Leiden Institute of Chemistry, Faculty of Science, Leiden University, ²Laboratory of Biophysics, Wageningen University & Research, ³Laboratory of BioNanoTechnology, Wageningen University & Research, ⁴MAGNEtic resonance Facility, Wageningen University & Research, ⁵C.J. Gorter Center for High Field MRI, Radiology department, Leiden University Medical Centre, Leiden University, ⁶Institute for Medical Physics and Biophysics, Leipzig University

A protocol to study biological tissue at high spatial resolution using ultra-high field magnetic resonance microscopy (MRM) using microcoils is presented. Step-by-step instructions are provided for characterizing the microcoils. Finally, optimization of imaging is demonstrated on plant roots.

Biochemistry

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Raphaël de Wijn^1,4, Kévin Rollet^1,2, Vincent Olieric³, Oliver Hennig², Nicola Thome¹, Camille Noûs¹, Caroline Paulus¹, Bernard Lorber¹, Heike Betat², Mario Mörl², Claude Sauter¹

¹Université de Strasbourg, Architecture et Réactivité de l’ARN, UPR 9002, CNRS, Institut de Biologie Moléculaire et Cellulaire, ²Biochemistry and Molecular Biology, Institute for Biochemistry, Leipzig University, ³Paul Scherrer Institute, Swiss Light Source, ⁴European XFEL GmbH

A versatile microfluidic device is described that enables the crystallization of an enzyme using the counter-diffusion method, the introduction of a substrate in the crystals by soaking, and the 3D structure determination of the enzyme:substrate complex by a serial analysis of crystals inside the chip at room temperature.