Atlanta Veterans Affairs Medical Center

4 ARTICLES PUBLISHED IN JoVE

Medicine

Transvaginal Mesh Insertion in the Ovine Model

Iva Urbankova^1,2, Geertje Callewaert^1,3, Nikhil Sindhwani¹, Alice Turri¹, Lucie Hympanova^1,2, Andrew Feola¹, Jan Deprest^1,3

¹Centrum for Surgical Technologies, Department of Development and Regeneration, Clinical Specialties Research Groups, Faculty of Medicine, KU Leuven, ²Institute for the Care of Mother and Child and Third Faculty of Medicine, Charles University, Prague, ³Pelvic Floor Unit, University Hospitals KU Leuven

This protocol describes mesh implantation in the ovine rectovaginal septum using a single vaginal incision technique, with and without the trocar-guided insertion of anchoring arms.

Bioengineering

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Rachael S. Allen^1,2, Katie Bales^1,3, Andrew Feola^1,2, Machelle T Pardue^1,2,3

¹Center of Excellence for Visual and Neurocognitive Rehabilitation, Atlanta Veterans Affairs Medical Center, ²Department of Biomedical Engineering, Georgia Institute of Technology, ³Department of Ophthalmology, Emory University

Here, we describe the use of spectral-domain optical coherence tomography (SD-OCT) to visualize retinal and ocular structures in vivo in models of retinal degeneration, glaucoma, diabetic retinopathy, and myopia.

Behavior

Behavioral Assessment of Visual Function via Optomotor Response and Cognitive Function via Y-Maze in Diabetic Rats

Kaavya Gudapati^*1,2, Anayesha Singh^*1,3, Danielle Clarkson-Townsend^1,4, Stephen Q. Phillips¹, Amber Douglass¹, Andrew J. Feola^1,2, Rachael S. Allen^1,2

¹Center for Visual and Neurocognitive Rehabilitation, Atlanta VA Medical Center, ²Department of Biomedical Engineering, Georgia Institute of Technology, ³Department of Neuroscience, Emory University, ⁴Gangarosa Department of Environmental Health, Emory University

Neural degeneration in both eyes and brain as a result of diabetes can be observed through behavioral tests carried out on rodents. The Y-maze, a measure of spatial cognition, and the optomotor response, a measure of visual function, both provide insight into potential diagnoses and treatments.

Biology

Streamlined Intravital Imaging Approach for Long-Term Monitoring of Epithelial Tissue Dynamics on an Inverted Confocal Microscope

Michael Hamersky IV¹, Khushi Tekale¹, L. Matthew Winfree², Matthew J. M. Rowan^1,3, Lindsey Seldin^1,4,5,6

¹Department of Cell Biology, Emory University School of Medicine, ²Independent scholar, ³Center for Neurodegenerative Disease, Emory University School of Medicine, ⁴Department of Dermatology, Emory University School of Medicine, ⁵Winship Cancer Institute, Emory University School of Medicine, ⁶Atlanta Veterans Affairs Medical Center

The protocol presents a new tool to simplify intravital imaging using inverted confocal microscopy.