Lineage Cell Therapeutics, Inc.

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Ratnesh K. Singh¹, Pablo E. Diaz¹, François Binette¹, Igor O. Nasonkin¹

¹BioTime, Inc

The objective of this report is to describe the protocol for robust immunohistochemical detection of epigenetic markers, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in developing and postmitotic mouse retina.

Neuroscience

Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model

Laurence M. Occelli^*1, Felipe Marinho^*1, Ratnesh K. Singh², Francois Binette², Igor O. Nasonkin², Simon M. Petersen-Jones¹

¹College of Veterinary Medicine, Department of Small Animal Clinical Sciences, Michigan State University, ²Lineage Cell Therapeutics, Inc.

Presented here is a surgical technique for transplanting human pluripotent stem cell (hPSC)-derived retinal tissue into the subretinal space of a large animal model.