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Lineage Cell Therapeutics, Inc.

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina
Ratnesh K. Singh 1, Pablo E. Diaz 1, François Binette 1, Igor O. Nasonkin 1
1BioTime, Inc

The objective of this report is to describe the protocol for robust immunohistochemical detection of epigenetic markers, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in developing and postmitotic mouse retina.

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Neuroscience

Subretinal Transplantation of Human Embryonic Stem Cell-Derived Retinal Tissue in a Feline Large Animal Model
Laurence M. Occelli *1, Felipe Marinho *1, Ratnesh K. Singh 2, Francois Binette 2, Igor O. Nasonkin 2, Simon M. Petersen-Jones 1
1College of Veterinary Medicine, Department of Small Animal Clinical Sciences, Michigan State University, 2Lineage Cell Therapeutics, Inc.

Presented here is a surgical technique for transplanting human pluripotent stem cell (hPSC)-derived retinal tissue into the subretinal space of a large animal model.

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