University of Turku, Turku, Finland
2 ARTICLES PUBLISHED IN JoVE
Developmental Biology
Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
Matteo Da Ros *1, Tiina Lehtiniemi *1, Opeyemi Olotu 1, Oliver Meikar 1, Noora Kotaja 1
1Institute of Biomedicine, University of Turku
Presented here is a protocol for enriching pachytene spermatocytes, round spermatids, and elongating spermatids from adult mouse testes using a discontinuous bovine serum albumin density gradient with standard laboratory equipment.
JoVE Core
Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
Juho-Antti Mäkelä 1, Sheyla Cisneros-Montalvo 1, Tiina Lehtiniemi 1, Opeyemi Olotu 1, Hue M. La 3,4, Jorma Toppari 1,2, Robin M. Hobbs 3,4, Martti Parvinen 1, Noora Kotaja 1
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, 2Department of Pediatrics, Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia
This protocol describes transillumination-assisted microdissection of segments of adult mouse seminiferous tubules representing specific stages of seminiferous epithelial cycle, and cell types therein, and subsequent immunostaining of squash preparations and intact tubule segments.
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