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University of Turku, Turku, Finland

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Matteo Da Ros^*1, Tiina Lehtiniemi^*1, Opeyemi Olotu¹, Oliver Meikar¹, Noora Kotaja¹

¹Institute of Biomedicine, University of Turku

Presented here is a protocol for enriching pachytene spermatocytes, round spermatids, and elongating spermatids from adult mouse testes using a discontinuous bovine serum albumin density gradient with standard laboratory equipment.

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Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses

Juho-Antti Mäkelä¹, Sheyla Cisneros-Montalvo¹, Tiina Lehtiniemi¹, Opeyemi Olotu¹, Hue M. La^3,4, Jorma Toppari^1,2, Robin M. Hobbs^3,4, Martti Parvinen¹, Noora Kotaja¹

¹Institute of Biomedicine, Integrative Physiology and Pharmacology Unit, University of Turku, Turku, Finland, ²Department of Pediatrics, Turku University Hospital, Turku, Finland, ³Centre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, ⁴Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia

This protocol describes transillumination-assisted microdissection of segments of adult mouse seminiferous tubules representing specific stages of seminiferous epithelial cycle, and cell types therein, and subsequent immunostaining of squash preparations and intact tubule segments.