Ralph H. Johnson Veterans Affairs Medical Center

3 ARTICLES PUBLISHED IN JoVE

Genetics

Expansion of Two-dimension Electrospun Nanofiber Mats into Three-dimension Scaffolds

Emily Keit¹, Shixuan Chen¹, Hongjun Wang¹, Jingwei Xie¹

¹Department of Surgery-Transplant and Mary & Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center

This article demonstrates the technique of expanding a traditional, two-dimension (2D) electrospun nanofiber mat into a three-dimension (3D) scaffold through the depressurization of subcritical CO₂ fluid. These augmented scaffolds are 3D, closely mimic cellular nanotopographic cues, and preserve the functions of biologic molecules encapsulated within the nanofibers.

Medicine

A Mouse Model for Chronic Pancreatitis via Bile Duct TNBS Infusion

Wenyu Gou^*1, Lindsay Swaby^*1, A. Marissa Wolfe², William P. Lancaster^1,2, Katherine A. Morgan¹, Hongjun Wang^1,2

¹Department of Surgery, Medical University of South Carolina, ²Ralph H. Johnson Veterans Affairs Medical Center

Chronic pancreatitis (CP) is a disease characterized by inflammation and fibrosis of the pancreas, often associated with intractable abdominal pain. This article focuses on refining the technique to generate a mouse model of CP via bile duct infusion with 2,4,6 -trinitrobenzene sulfonic acid (TNBS).

Medicine

Minimizing Post-Infusion Portal Vein Bleeding during Intrahepatic Islet Transplantation in Mice

Wenyu Gou^*1, Wanxing Cui^*2, Yuki Cui², Hongjun Wang^1,3

¹Department of Surgery, Medical University of South Carolina, ²Georgetown University, ³Ralph H. Johnson Veterans Affairs Medical Center

Here we present refined surgical procedures on successfully performing intraportal islet transplantation, a clinically relevant but technically challenging surgical procedure, in mice.